Abstract

Conversion of adenosine to inosine is a frequent type of RNA editing, but important details about its biology remain unknown due to a lack of imaging tools. We developed inoFISH to directly visualize and quantify adenosine-to-inosine edited transcripts in situ. We found that editing of GRIA2, EIF2AK2, and NUP43 is uncorrelated with nuclear localization and paraspeckle association. Further, NUP43 exhibits constant editing levels between single cells while GRIA2 levels vary.